Plasticity of Calcium Signaling Cascades in Human Embryonic Stem Cell-Derived Neural Precursors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F13%3A10209663" target="_blank" >RIV/00216208:11130/13:10209663 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/13:00396604
Result on the web
<a href="http://dx.doi.org/10.1089/scd.2012.0624" target="_blank" >http://dx.doi.org/10.1089/scd.2012.0624</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/scd.2012.0624" target="_blank" >10.1089/scd.2012.0624</a>
Alternative languages
Result language
angličtina
Original language name
Plasticity of Calcium Signaling Cascades in Human Embryonic Stem Cell-Derived Neural Precursors
Original language description
Human embryonic stem cell-derived neural precursors (hESC NPs) are considered to be a promising tool for cell-based therapy in central nervous system injuries and neurodegenerative diseases. The Ca2+ ion is an important intracellular messenger essentialfor the regulation of various cellular functions. We investigated the role and physiology of Ca2+ signaling to characterize the functional properties of CCTL14 hESC NPs during long-term maintenance in culture (in vitro). We analyzed changes in cytoplasmic Ca2+ concentration ([Ca2+](i)) evoked by high K+, adenosine-5'-triphosphate (ATP), glutamate, gamma-aminobutyric acid (GABA), and caffeine in correlation with the expression of various neuronal markers in different passages (P6 through P10) during thecourse of hESC differentiation. We found that only differentiated NPs from P7 exhibited significant and specific [Ca2+](i) responses to various stimuli. About 31% of neuronal-like P7 NPs exhibited spontaneous [Ca2+](i) oscillations. Pharm
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Stem Cells and Development
ISSN
1547-3287
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
1506-1521
UT code for WoS article
000318684900004
EID of the result in the Scopus database
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