Efficacy and Safety of Human Mesenchymal Stromal Cells in Healing of Critical-Size Bone Defects in Immunodeficient Rats

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10360752" target="_blank" >RIV/00216208:11130/17:10360752 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/17:10360752 RIV/00064203:_____/17:10360752 RIV/00216208:11110/17:10360752 RIV/00064165:_____/17:10360752
Result on the web
<a href="http://www.biomed.cas.cz/physiolres/pdf/66/66_113.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/66/66_113.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Efficacy and Safety of Human Mesenchymal Stromal Cells in Healing of Critical-Size Bone Defects in Immunodeficient Rats
Original language description
To evaluate the preclinical efficacy and safety of human mesenchymal stem cells (hMSC) rapidly expanded in growth medium for clinical use with human serum and recombinant growth factors, we conducted a controlled, randomized trial of plasma clots with hMSC vs. plasma clots only in critical segmental femoral defects in rnu/rnu immunodeficient rats. X-ray, microCT and histomorphometrical evaluation were performed at 8 and 16 weeks. MSC were obtained from healthy volunteers and patients with lymphoid malignancy. Human MSC survived in the defect for the entire duration of the trial. MSC from healthy volunteers, in contrast to hMSC from cancer patients, significantly improved bone healing at 8, but not 16 weeks. However, at 16 weeks, hMSC significantly improved vasculogenesis in residual defect. We conclude that hMSC from healthy donors significantly contributed to the healing of bone defects at 8 weeks and to the vascularisation of residual connective tissue for up to 16 weeks. We found the administration of hMSC to be safe, as no adverse reaction to human cells at the site of implantation and no evidence of migration of hMSC to distant organs was detected.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)

Project
<a href="/en/project/NT13531" target="_blank" >NT13531: Human mesenchymal stromal cells for therapeutic purposes. Preclinical and pharmacological testing and approval of the cellular product for clinical use.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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