Nitric Oxide Elevation in Polytrauma is Driven by Oxygen Radicals

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10369386" target="_blank" >RIV/00216208:11130/17:10369386 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/17:10369386

Result on the web

<a href="http://www.biomed.cas.cz/physiolres/pdf/66/66_S561.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/66/66_S561.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nitric Oxide Elevation in Polytrauma is Driven by Oxygen Radicals

Original language description

A common problem in management of polytrauma - a simultaneous injury to more than one organ or organ system, at least one of them lethal without intervention - is a discrepancy between a relatively good initial state and a serious subsequent development. Since nitric oxide (NO) is produced in high quantities during tissue injury, we assumed that serum levels of NO (and its oxidation products, NOx) might serve as a prognostic marker of polytrauma severity. However, we found recently that NOx was increased in polytrauma, but not in the most severe cases. The present study was undertaken to test the hypothesis that serum NOx is reduced in severe polytrauma by concomitant overproduction of reactive oxygen species (ROS). Polytrauma was induced in rats under anesthesia by bilateral fracture of femurs and tibiae plus incision of the right liver lobe through laparotomy. Serum NOx was measured by chemiluminescence after hot acidic reduction. The role of ROS was assessed by treatment with an antioxidant, N-acetyl-L-cysteine (NAC). Experimental polytrauma elevated NOx from 11.0 +/- 0.7 to 23.8 +/- 4.5 ppb. This was completely prevented by NAC treatment (9.1 +/- 2.2 ppb). Serum NOx is elevated in severe polytrauma, and this is not reduced by ROS. On the contrary, ROS are necessary for the NOx elevation, probably because ROS produced by inflammatory cells activated by the polytrauma induce massive NO production.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30105 - Physiology (including cytology)

Project

<a href="/en/project/GA13-01710S" target="_blank" >GA13-01710S: Vascular reactivity in pulmonary hypertension</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

66

Issue of the periodical within the volume

Supplement 4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

"S561"-"S565"

UT code for WoS article

000422901400015

EID of the result in the Scopus database

2-s2.0-85041715274