Appearance of cytomegalovirus-specific T-cells predicts fast resolution of viremia post hematopoietic stem cell transplantation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F17%3A10373644" target="_blank" >RIV/00216208:11130/17:10373644 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/17:10373644
Result on the web
<a href="https://doi.org/10.1002/cyto.b.21348" target="_blank" >https://doi.org/10.1002/cyto.b.21348</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.b.21348" target="_blank" >10.1002/cyto.b.21348</a>
Alternative languages
Result language
angličtina
Original language name
Appearance of cytomegalovirus-specific T-cells predicts fast resolution of viremia post hematopoietic stem cell transplantation
Original language description
BackgroundCytomegalovirus (CMV) specific T-cells are known to provide long-term control of CMV reactivation, which is a frequent complication of hematopoietic stem cell transplantation. We have studied 58 pediatric patients after hematopoietic stem cell transplantation who suffered from CMV reactivation to reveal which functional T cell subset is best correlating with successful reactivation resolution and which protects from reactivation episode. MethodsDetection of 30 combinatorial subsets of four types of response to ex vivo CMV stimulation (IFN secretion, IL-2 secretion, CD40L upregulation and degranulation) that were detectable on either CD8+ or CD4+ T cells through flow cytometry intracellular cytokine staining was used. ResultsWe found that the presence of CD8+ dual positive (IFN+ and IL-2+) cells is the most accurate functional parameter that can predict fast resolution of CMV reactivation. Next, we show that the presence of CD8+ dual positive (IFN+ and IL-2+) and CD8+ IFN+ cells provides a protective effect (a hazard risk of 0.28 (confidence interval 0.18 - 0.43) and 0.45 (CI 0.27 - 0.75), respectively) and the presence of corticotherapy increases the risk of reactivation (HR 2.47 (CI 1.82-3.36)). Thus, a patient without corticotherapy and with both of the critical T cell subsets present has a cumulative 19.6 times lower risk of developing CMV reactivation than a patient on corticotherapy and without CD8+ dual positive (IFN+ and IL-2+) or CD8+ IFN+ cells. ConclusionsWe have established parameters of CMV specific functional response ex vivo that can be used in assisting clinical management of patients with CMV reactivation. (c) 2015 International Clinical Cytometry Society
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
<a href="/en/project/GA13-22777S" target="_blank" >GA13-22777S: Adoptive transfer of CMV specific CD8+ T-cells to treat CMV infection after transplantation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytometry Part B - Clinical Cytometry
ISSN
1552-4949
e-ISSN
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Volume of the periodical
92
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
380-389
UT code for WoS article
000409938500008
EID of the result in the Scopus database
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