The effect of exposure to hypoxia on superoxide formation by alveolar macrophages is indirect
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F19%3A10399663" target="_blank" >RIV/00216208:11130/19:10399663 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=tyOz9NeWYU" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=tyOz9NeWYU</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.lfs.2019.116864" target="_blank" >10.1016/j.lfs.2019.116864</a>
Alternative languages
Result language
angličtina
Original language name
The effect of exposure to hypoxia on superoxide formation by alveolar macrophages is indirect
Original language description
Aims: To elucidate the role of alveolar macrophages (AM) in the pathogenesis of hypoxic pulmonary hypertension (HPH), we tested the effects of sustained hypoxia on AM polarization and on the formation of superoxide by AM in vivo and in vitro. Main methods: Rat AM were obtained by bronchoalveolar lavage. 4-day exposure to hypoxia (10% O2) was carried out in vivo (rats in isobaric hypoxic chamber, controls kept in air) or in vitro (control AM in 21% O2 and 5% CO2). Superoxide production was measured by luminol-orthovanadate chemiluminescence, AM polarization was detected immunocytochemically. To ascertain the effect of substances contained in the alveolar environment, we cultivated cells also in the presence of non-cellular components of the bronchoalveolar lavage fluid (BALF) either from controls or from rats exposed to 4 days of hypoxia. Key findings: In vivo, but not in vitro, hypoxia increased AM superoxide production. Both types of hypoxia polarized AM into M2 (pro-proliferative) type. While the presence of control BALF attenuated superoxide production in AM cultivated in normoxia, BALF from the hypoxia-exposed rats had no effect. In AM cultivated in hypoxia, superoxide production was not altered by control BALF and elevated by BALF obtained from hypoxic rats. Significance: Hypoxia does not influence superoxide production by AM directly but rather by modulating their milieu and their sensitivity to external influences. (C) 2019 Elsevier Inc.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
<a href="/en/project/GA17-11223S" target="_blank" >GA17-11223S: ONTOGENETIC MECHANISMS THAT DETERMINE PUMONARY VASCULAR DISEASE IN ADULTS</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Life Sciences
ISSN
0024-3205
e-ISSN
—
Volume of the periodical
236
Issue of the periodical within the volume
November
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
116864
UT code for WoS article
000492349800018
EID of the result in the Scopus database
2-s2.0-85072852721