Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F20%3A10411271" target="_blank" >RIV/00216208:11130/20:10411271 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/20:00116334 RIV/65269705:_____/20:00072820 RIV/00064203:_____/20:10411271
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=cEkRQ5Ct3~" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=cEkRQ5Ct3~</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/ppul.24758" target="_blank" >10.1002/ppul.24758</a>
Alternative languages
Result language
angličtina
Original language name
Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis
Original language description
Objective: To assess the performance of a newly developed skin wipe test (SWT) for the diagnosis of cystic fibrosis (CF). Study Design: Spontaneously formed sweat from the forearm was wiped by a cotton swab moistened with 100 µL of deionized (DI) water and extracted into 400 µL of DI water (SWT). The conventional Macroduct sweat test (ST) was performed simultaneously. SWT samples of 114 CF patients, 76 healthy carriers, and 58 controls were analyzed by capillary electrophoresis with contactless conductivity detection and Cl-/K+ and (Cl- + Na+)/K+ ion ratios were evaluated. Chloride concentrations from Macroduct ST were analyzed coulometrically. Results: Analysis of 248 SWT samples and simultaneous Macroduct ST samples showed comparable method performance. Two ion ratios, Cl-/K+ and (Cl- + Na+)/K+, from the SWT samples and Cl- values from the ST samples were evaluated to diagnose CF. Sensitivity of the SWT method using the Cl-/K+ ratio (cutoff value 3.9) was 93.9%, compared to 99.1% when using the (Cl- + Na+)/K+ ratio (cutoff value 5.0) and 98.3% in using Macroduct Cl- (cutoff value higher or equal to 60 mmol/L). The methods' specificities were 97.8%, 94.0%, and 100.0%, respectively. Conclusions: The developed SWT method with capillary electrophoretic analysis for CF diagnosis performs comparably to the conventional Macroduct ST. The SWT method is simple, fast, inexpensive, and completely noninvasive. Use of an ion ratio in obtained SWT samples is proposed as a new diagnostic parameter that shows significant promise in CF diagnostics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30209 - Paediatrics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Pediatric Pulmonology
ISSN
8755-6863
e-ISSN
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Volume of the periodical
55
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
1653-1660
UT code for WoS article
000559584700001
EID of the result in the Scopus database
2-s2.0-85085076847