Skin-wipe sweat samples: sensitivity and specificity of (Cl-+Na+)/K+ratio

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00070811" target="_blank" >RIV/65269705:_____/19:00070811 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/19:00108584

Result on the web

<a href="https://www.cysticfibrosisjournal.com/article/S1569-1993(19)30332-7/fulltext" target="_blank" >https://www.cysticfibrosisjournal.com/article/S1569-1993(19)30332-7/fulltext</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Skin-wipe sweat samples: sensitivity and specificity of (Cl-+Na+)/K+ratio

Original language description

Objectives: Ion ratio in sweat sample obtained by skin-wipe test and analysed by capillary electrophoresis with contactless conductometric detection appears to be a promising alternative to the usual sweat test. The objective of the study was to determine the cut-off value of ion ratio that can reliably differentiate between CF patients and healthy individuals. Methods: The sampling consists of wiping 10 cm2 forearm skin with a pre-cleaned cotton swab moistened with 100 µL deionized water, then extracted for 3 min in 400 µL deionized water. The extract is analyzed simultaneously by capillary electrophoresis with contactless conductometric detection using a double opposite end injection. Ion ratio (Cl-+Na+)/K+ was established for CF patients and healthy controls and cut off value was determined. At the same time, the standard sweat test was performed on the other forearm (Macroduct). Several CF patients underwent longitudinal skin-wipe testing to establish individual variability of ion ratio in time. Study population: 160 patients, age 0-18 years, 65 CF patients (sweat chloride GREATER-THAN OR EQUAL TO60 mmol/l and two causing CFTR mutations), 95 healthy controls (sweat chloride &lt; 30 mmol/l) including 46 carriers of 1 CFTR mutation. Results: By determining cut-off value = 5.0 for the ion ratio (Cl-+Na+)/K+ we have reached 100% sensitivity of the skin-wipe test, overall specificity was 93%, specificity for healthy individuals was 96%, specificity for carriers was 89%. Despite its individual variability, all the measurements were above the cut-off value in longitudinal monitoring in CF patients. Conclusion: The skin-wipe sweat sampling is a fast, simple, cheap and painless method that can reliably identify CF patients (Cl-+Na+)/K+ cut-off 5.0, sensitivity 100%, specificity 93%. Therefore it appears to be a usable screening method.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

30200 - Clinical medicine

Project

<a href="/en/project/NV18-08-00189" target="_blank" >NV18-08-00189: New approach for diagnosis of cystic fibrosis based on ion ratios in noninvasively obtained samples</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů