How to: screening for mcr-mediated resistance to colistin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F22%3A10432138" target="_blank" >RIV/00216208:11130/22:10432138 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/22:10432138
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FXY.ozKI5f" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FXY.ozKI5f</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cmi.2021.09.009" target="_blank" >10.1016/j.cmi.2021.09.009</a>
Alternative languages
Result language
angličtina
Original language name
How to: screening for mcr-mediated resistance to colistin
Original language description
BACKGROUND: Colistin belongs to the last-resort antibiotics. The discovery of a plasmid-bound colistin resistance mediated by the mcr-gene(s) is of great concern since, given its biological potential, there is a risk of its rapid spread. OBJECTIVES: To discuss the current literature on the methods for the screening of mcr-mediated resistance to colistin. SOURCES: Literature was drawn from a search of PubMed between 1 January 2016 to 26 April 2021. CONTENT: The selective culture-based or culture-independent approach can be used for the screening of mcr-mediated resistance to colistin in clinical samples. Rapid Polymyxin NP, Colistin Drop or Colistin Agar Spot tests are applicable for the selection of isolates with a suspected resistance to colistin that has to be confimed by broth microdilution. The mcr-mediated resistance to colistin can be confirmed by the detection of the causal gene(s) or by phenotype using the EDTA-colistin broth disk elution; the production of the MCR-1 enzyme can be confirmed with lateral flow immunoassay, using MALDI-TOF or Liquid chromatography-based mass spectrometry. Whole genome sequencing (WGS) is the ultimate typing method. When a WGS platform is not available at a healthcare facility, a WGS-outsourced service, in combination with freely available bioinformatics tools, allows for the characterisation of the mcr-gene(s) carrying isolates. IMPLICATIONS: Mcr-mediated colistin resistance should be monitored through active targeted screening.The broth microdilution method is required for colistin susceptibility testing but as only a selected number of clinical isolates are tested, colistin resistance, including mcr-mediated, may remain undetected. In mcr-1 positive E. coli isolates, the minimal inhibitory concentration (MIC) to colistin can range from 2-8 mg/L, thus it is proposed that Enterobacterales with a colistin MIC of 2 mg/L should be also included in the mcr-mediated colistin resistance screening and those with a confirmed mcr-genotype and/or MCR-phenotype should be considered to be colistin-resistant.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
<a href="/en/project/NV18-09-00254" target="_blank" >NV18-09-00254: Sentinel testing survey of plasmid-mediated (transferable) colistin resistance among Enterobacteriaceae in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Microbiology and Infection
ISSN
1198-743X
e-ISSN
1469-0691
Volume of the periodical
28
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
43-50
UT code for WoS article
000735207100007
EID of the result in the Scopus database
2-s2.0-85121445328