Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F24%3A10489593" target="_blank" >RIV/00216208:11130/24:10489593 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10489593
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=4oqwi_1CK1" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=4oqwi_1CK1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/forensicsci4040038" target="_blank" >10.3390/forensicsci4040038</a>
Alternative languages
Result language
angličtina
Original language name
Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory
Original language description
In scenarios when the morphological identification of samples is not feasible, the utilization of molecular analyses becomes an imperative. However, that can often be challenging in conditions where standard molecular laboratories cannot be established. In this study, we present a simplified and field-friendly protocol of the previously published Triplex PCR Assay for the identification of mammalian and plant sample origin using a fully portable molecular laboratory from Bento Lab (Bento Bioworks Ltd., London, UK), effectively eliminating the need for a traditional laboratory setup. The protocol in combination with correctly selected kits enables DNA extraction, result evaluation based on electrophoresis, and direct use of the PCR products for downstream analyses such as Sanger sequencing and third-generation sequencing; therefore, enabling the use of molecular analysis directly in situ or for educational purposes in a classroom.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10511 - Environmental sciences (social aspects to be 5.7)
Result continuities
Project
<a href="/en/project/VJ01010026" target="_blank" >VJ01010026: Effective use of forensic evidence methods to combat wildlife crime</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Forensic Sciences (Basel)
ISSN
2673-6756
e-ISSN
2673-6756
Volume of the periodical
4
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
7
Pages from-to
566-572
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85213495933