Pixe maps of intracellular element distribution in cerebellar neurons

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F12%3A10125478" target="_blank" >RIV/00216208:11140/12:10125478 - isvavai.cz</a>

Alternative codes found

RIV/61389005:_____/12:00388232

Result on the web

<a href="http://dx.doi.org/10.1142/S0129083512400190" target="_blank" >http://dx.doi.org/10.1142/S0129083512400190</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1142/S0129083512400190" target="_blank" >10.1142/S0129083512400190</a>

Result language

angličtina

Original language name

Pixe maps of intracellular element distribution in cerebellar neurons

Original language description

Neurodegenerative disorders are accompanied by altered compositions of metals that may convey some information about the health and possibly metabolic state of neurons. Here we opted for PIXE technique to perform a spatially-resolved elemental analysis of intracellular space of individual neurons. Primarily, we measured element distribution within Purkinje cells (PC) of normal and Lurcher (Lc/+) mutant mice to detect any anomalies in concentrations of individual elements attributable to cell death. Lc/+mutant mice provide an excellent animal model for time-course of excitotoxic cell death. PCs, some with autofluorescence, were scanned with a proton beam to record their X-ray emission and RBS spectra and to construct 2D-concentration maps of individualelements. PCs with auto-fluorescence, presumed to be either already dead or moribund, showed elevated concentrations of Cu, Fe, and Zn, when compared to healthy PCs. In conclusion, excitotoxic mode of death, as observed in the moribund P

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

<a href="/en/project/GA309%2F09%2F1189" target="_blank" >GA309/09/1189: Elemental analysis of intracellular structures of neurones.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of PIXE

ISSN

0129-0835

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

1a2

Country of publishing house

JP - JAPAN

Number of pages

8

Pages from-to

65-72

UT code for WoS article

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EID of the result in the Scopus database

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