Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F14%3A10284523" target="_blank" >RIV/00216208:11140/14:10284523 - isvavai.cz</a>
Alternative codes found
RIV/49777513:23520/14:43923974
Result on the web
<a href="http://www.engmech.cz/2014/im/doc/EM2014_proceedings.pdf" target="_blank" >http://www.engmech.cz/2014/im/doc/EM2014_proceedings.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections
Original language description
Aims of our study were as follows: 1) to perform a 3D reconstruction of parts of the adjacent classical hepatic lobules, 2) to compare the histomorphometric techniques available for estimating the volume of the lobules using 3D reconstruction and interactive stereological techniques, 3) to assess the volume fraction of liver parenchyma, connective tissue, and blood vessels, 4) to quantify the shrinkage in tissue blocks porcine liver. The morphometric parameters were assessed using serial histological sections and stereological methods. Volume of the sampled portions of the lobules ranged between 0.282-0.498 mm3. The volume fraction of parenchyma in liver was 0.867, the volume fraction of connective tissue was 0.077and the fraction of blood vessels was 0.026. The mean volume shrinkage of porcine liver was 55.23%. This value can be used for correcting the data based on paraffin-processed histological sections. For further quantitative studies, we suggest the nucleator technique as a fast, robust and reliable method for estimating the volume of the lobules. For biomechanical modelling, the absolute volumes resulting from histological studies have to be corrected due to the tissue shrinkage affecting the paraffin-processed tissue samples.
Czech name
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Czech description
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Project
<a href="/en/project/ED2.1.00%2F03.0076" target="_blank" >ED2.1.00/03.0076: Biomedical Centre of the Faculty of Medicine in Pilsen</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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