Stereological analysis of size and density of hepatocytes in the porcine liver

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49777513%3A23520%2F17%3A43931521" target="_blank" >RIV/49777513:23520/17:43931521 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11140/17:10333635

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1111/joa.12585/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/joa.12585/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/joa.12585" target="_blank" >10.1111/joa.12585</a>

Result language

angličtina

Original language name

Stereological analysis of size and density of hepatocytes in the porcine liver

Original language description

The porcine liver is frequently used as a large animal model for verification of surgical techniques, as well as experimental therapies. Often, a histological evaluation is required that include measurements of the size, nuclearity or density of hepatocytes. Our aims were to assess the mean number-weighted volume of hepatocytes, the numerical density of hepatocytes, and the fraction of binuclear hepatocytes (BnHEP) in the porcine liver, and compare the distribution of these parameters among hepatic lobes and macroscopic regions of interest (ROIs) with different positions related to the liver vasculature. Using disector and nucleator as design-based stereological methods, the morphometry of hepatocytes was quantified in seven healthy piglets. The samples were obtained from all six hepatic lobes and three ROIs (peripheral, paracaval and paraportal) within each lobe. Histological sections (thickness 16 lm) of formalin-fixed paraffin-embedded material were stained with the periodic acid-Schiff reaction to indicate the cell outlines and were assessed in a series of 3-lm-thick optical sections. The mean number-weighted volume of mononuclear hepatocytes (MnHEP) in all samples was 3670  805 lm3 (mean  SD). The mean number-weighted volume of BnHEP was 7050  2550 lm3. The fraction of BnHEP was 4  2%. The numerical density of all hepatocytes was 146 997  15 738 cells mm 3 of liver parenchyma. The porcine hepatic lobes contained hepatocytes of a comparable size, nuclearity and density. No significant differences were identified between the lobes. The peripheral ROIs of the hepatic lobes contained the largest MnHEP with the smallest numerical density. The distribution of a larger MnHEP was correlated with a larger volume of BnHEP and a smaller numerical density of all hepatocytes. Practical recommendations for designing studies that involve stereological evaluations of the size, nuclearity and density of hepatocytes in porcine liver are provided.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF ANATOMY

ISSN

0021-8782

e-ISSN

—

Volume of the periodical

230

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

575-588

UT code for WoS article

000398042300008

EID of the result in the Scopus database

2-s2.0-85007453501