Distribution of Connective Tissue in the Male and Female Porcine Liver: Histological Mapping and Recommendations for Sampling

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F18%3A10376356" target="_blank" >RIV/00216208:11140/18:10376356 - isvavai.cz</a>

Alternative codes found

RIV/49777513:23520/18:43951680

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0021997518300720" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021997518300720</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jcpa.2018.05.004" target="_blank" >10.1016/j.jcpa.2018.05.004</a>

Result language

angličtina

Original language name

Distribution of Connective Tissue in the Male and Female Porcine Liver: Histological Mapping and Recommendations for Sampling

Original language description

The pig is a large animal model that is often used in experimental medicine. The aim of this study was to assess, in normal pig livers, sexual dimorphism in the normal fraction of hepatic interlobular and intralobular connective tissue (CT) in six hepatic lobes and in three macroscopical regions of interest (ROIs) with different positions relative to the liver vasculature. Using stereological point grids, the fractions of CT were quantified in histological sections stained with aniline blue and nuclear fast red. Samples (415 tissue blocks) were collected from healthy piglets, representing paracaval, paraportal and peripheral ROIs. There was considerable variability in the CT fraction at all sampling levels. In males the mean fraction of interlobular CT was 4.7 +- 2.4% (mean +- SD) and ranged from 0% to 11.4%. In females the mean fraction of the interlobular CT was 3.6 +- 2.2% and ranged from 0% to 12.3%. The mean fraction of intralobular (perisinusoidal summed with pericentral) CT was &lt;0.2% in both sexes. The interlobular CT represented &gt;99.8% of the total hepatic CT and the fractions were highly correlated (Spearman r = 0.998, P &lt;0.05). The smallest CT fraction was observed in the left medial lobe and in the paracaval ROI and the largest CT fraction was detected in the quadrate lobe and in the peripheral ROI. For planning experiments involving the histological quantification of liver fibrosis and requiring comparison between the liver lobes, these data facilitate the power analysis for sample size needed to detect the expected relative increase or decrease in the fraction of CT.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30106 - Anatomy and morphology (plant science to be 1.6)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Comparative Pathology

ISSN

0021-9975

e-ISSN

—

Volume of the periodical

162

Issue of the periodical within the volume

july

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

1-13

UT code for WoS article

000441655400001

EID of the result in the Scopus database

2-s2.0-85049106097