Oestrous sheep serum balances ROS levels to supply in vitro capacitation of ram spermatozoa
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F16%3A10324637" target="_blank" >RIV/00216208:11140/16:10324637 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/rda.12741" target="_blank" >http://dx.doi.org/10.1111/rda.12741</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/rda.12741" target="_blank" >10.1111/rda.12741</a>
Alternative languages
Result language
angličtina
Original language name
Oestrous sheep serum balances ROS levels to supply in vitro capacitation of ram spermatozoa
Original language description
Reactive oxygen species (ROS) are fundamental for intracellular signaling. In spermatozoa, they are involved both to apoptosis and to capacitation, and changes in ROS levels can alter the balance between these two processes. Estrous sheep serum (ESS) is considered an efficient agent for in vitro capacitation of ram spermatozoa. We have explored the effects of ESS on ram sperm physiology, especially on ROS production, during in vitro capacitation. Semen samples from 15 rams were cryopreserved. After thawing, samples were submitted to four treatments: Control (CTL), 10% ESS supplementation for in vitro sperm capacitation, caspase inhibitor (INH, Z-VAD-FMK 100 μM), and EES (10%) plus caspase inhibitor (I+E). Sperm samples were incubated for 30 min at 38.5 oC and 5% CO2 and evaluated: motility and kinetic parameters by computer-assisted semen analysis (CASA), and viability (propidium iodide), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), intracellular calcium (FLUO-3), membrane fluidity (M540) and ROS production (CM-H2DCFDA) by flow cytometry. ESS induced changes in kinetic parameters compatible with capacitation, with a decrease in the percentage of progressive motility and linearity, and an increase in the amplitude of the lateral displacement of the sperm head (P<0.05). Moreover, ESS increased the proportion of M540+ viable spermatozoa, YO-PRO-1+ and acrosome reacted spermatozoa (P<0.05). After incubation, ESS and I+E achieved lower ROS levels (P < 0.05). Ca2+ levels did not change with the incubation, but were slightly higher (P<0.05) when both ESS and the inhibitor were present. We suggest that ESS may modulate ROS levels, allowing intracellular signalling for capacitation to occur while preventing higher levels that could trigger apoptosis.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reproduction in Domestic Animals
ISSN
0936-6768
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
743-750
UT code for WoS article
000388334100014
EID of the result in the Scopus database
2-s2.0-84986910751