Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26788462%3A_____%2F11%3A%230000536" target="_blank" >RIV/26788462:_____/11:#0000536 - isvavai.cz</a>
Alternative codes found
RIV/49608851:_____/11:#0000495
Result on the web
<a href="http://dx.doi.org/10.4149/gpb_2011_SI1_36" target="_blank" >http://dx.doi.org/10.4149/gpb_2011_SI1_36</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4149/gpb_2011_SI1_36" target="_blank" >10.4149/gpb_2011_SI1_36</a>
Alternative languages
Result language
angličtina
Original language name
Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor
Original language description
The aim of the study was to examine the effects of epidermal growth factor (EGF) on ram sperm traits following hypothermic storage. Fresh ram ejaculates were diluted in Triladyl extender, pooled and divided into groups according to EGF doses added (0, 100,200 or 400 ng/ml). Following 72-96 h storage at 4 degrees C, the spermatozoa were stained for a plasma membrane integrity (PNA-FITC), membrane phosphatidylserine (PS) translocation (annexin V-Fluos) and apoptosis (Yo-Pro-1), and analyzed by fluorescentmicroscopy. Sperm motility was measured using computer-assisted sperm analysis (CASA) and sperm fertilizing ability was tested using in vitro penetration/fertilization test on bovine prematured oocytes. EGF increased sperm motility at all doses tested,decreased the proportion of spermatozoa with damaged plasma membrane (at 200 or 400 ng/ml), and decreased the proportion of apoptotic (Yo-Pro-1) spermatozoa when given at 200 or 400 (but not 100) ng/ml. The proportion of spermatozoa with
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GG - Zootechnics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QH81324" target="_blank" >QH81324: Development and utilization of biotechnology methods in reproduction small ruminants.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
GENERAL PHYSIOLOGY AND BIOPHYSICS
ISSN
0231-5882
e-ISSN
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Volume of the periodical
30
Issue of the periodical within the volume
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Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
36-43
UT code for WoS article
000293112300005
EID of the result in the Scopus database
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