Gentamicin preserves motility and viability of Zebrafish (Danio rerio) sperm inoculated with bacteria during short-term storage
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43910051" target="_blank" >RIV/60076658:12520/25:43910051 - isvavai.cz</a>
Result on the web
<a href="http://jifro.ir/article-1-5695-fa.html" target="_blank" >http://jifro.ir/article-1-5695-fa.html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.22092/ijfs.2025.133805" target="_blank" >10.22092/ijfs.2025.133805</a>
Alternative languages
Result language
angličtina
Original language name
Gentamicin preserves motility and viability of Zebrafish (Danio rerio) sperm inoculated with bacteria during short-term storage
Original language description
Sperm short-term storage at low temperatures provides an advantage to control artificial reproduction in fish. However, spermatozoa lose their potential for motility and fertilization during the storage period that may be associated with microbial growth. In the present study, we used Zebrafish (Danio rerio) sperm to investigate the effects of bacterial contamination on spermatozoa motility and viability during short-term storage. Spermatozoa retained better motility characteristics during the storage period when sperm was diluted with an extender compared to the undiluted sperm. Inoculation of sperm with bacteria decreased spermatozoa motility in a concentration-dependent manner. Through the storage period, spermatozoa motility was not affected in sperm inoculated with 103 CFU/mL Vibrio harveyi or Pseudomonas aeruginosa. A significant decrease in spermatozoa motility was observed in the sperm inoculated with 108 CFU/mL V. harveyi and with 105 CFU/mL P. aeruginosa at 3 h post-storage. Next, we determined the effective concentrations of gentamicin to preserve spermatozoa against bacteria during short-term storage. The spermatozoa motility was similar to that of non-infected sperm when 0.08-0.15 and 0.02-0.04 mg/mL gentamicin was added to the sperm inoculated with 108 CFU/mL V. harveyi or P. aeruginosa, respectively. At 24 h post-storage, spermatozoa viability was significantly decreased in the sperm inoculated with 108 CFU/mL V. harveyi or P. aeruginosa, while it showed an increasing trend of spermatozoa viability in bacterially infected sperm with gentamicin. The results showed the detrimental effects of bacteria on spermatozoa motility during short-term storage, and determined effective dose of gentamicin to preserve spermatozoa against V. harveyi or P. aeruginosa. This study provides valuable information to develop artificial in Zebrafish research centers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40103 - Fishery
Result continuities
Project
<a href="/en/project/QK21010141" target="_blank" >QK21010141: Management of gametes and reproduction in the protection of genetic resources and the breeding program of common carp in the conditions of aquaculture in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Iranian Journal of Fisheries Sciences
ISSN
1562-2916
e-ISSN
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Volume of the periodical
24
Issue of the periodical within the volume
3
Country of publishing house
IR - IRAN, ISLAMIC REPUBLIC OF
Number of pages
17
Pages from-to
633-649
UT code for WoS article
001524077500012
EID of the result in the Scopus database
2-s2.0-105006693924