Transferrin maintains the motility rate, ATP content, and DNA integrity of common carp spermatozoa during short-term storage
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00585279" target="_blank" >RIV/61388971:_____/24:00585279 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12520/24:43908094 RIV/00216208:11310/24:10479661
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0378432024000289?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378432024000289?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.anireprosci.2024.107437" target="_blank" >10.1016/j.anireprosci.2024.107437</a>
Alternative languages
Result language
angličtina
Original language name
Transferrin maintains the motility rate, ATP content, and DNA integrity of common carp spermatozoa during short-term storage
Original language description
Short-term sperm storage is a straightforward and cost-effective method of managing logistics in large scale fish hatchery operations but may result in decline in sperm quality. For effective artificial reproduction of fish, use of an appropriate additive to optimize sperm storage conditions is essential. In this study, it was investigated the effect of purified seminal plasma transferrin (Tf) at 10 mu g/ml on relevant parameters in common carp Cyprinus carpio sperm during short-term storage. We compared sperm motility and curvilinear velocity, adenosine triphosphate (ATP) content and DNA fragmentation of fresh spermatozoa to that stored for 24, 48, 72, and 144 h with or without Tf. The percentage of motile cells and the curvilinear velocity of spermatozoa in stored samples for 72 h with transferrin supplementation were greater compared to samples with no added protein. The ATP content in samples without added transferrin was reduced (P < 0.05) after 72 h of storage, in contrast to the levels observed in transferrin-supplemented sperm. A time-dependent increase in DNA fragmentation was observed. Significantly lower DNA damage, expressed as percent tail DNA (10.99 +/- 1.28) and olive tail moment (0.54 +/- 0.12), was recorded in Tf-supplemented samples stored for 48 h compared to that with no Tf. Hence, it is concluded that the beneficial effects of transferrin on common carp sperm could serve as an additional tool for developing and enhancing short-term sperm preservation procedures commonly used in aquaculture.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
<a href="/en/project/EF16_025%2F0007370" target="_blank" >EF16_025/0007370: Reproductive and genetic procedures for preserving fish biodiversity and aquaculture</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animal Reproduction Science
ISSN
0378-4320
e-ISSN
1873-2232
Volume of the periodical
263
Issue of the periodical within the volume
April 2024
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
107437
UT code for WoS article
001197801500001
EID of the result in the Scopus database
2-s2.0-85185801305