Effect of sex-sorting and cryopreservation on the post-thaw sperm quality of Iberian red deer spermatozoa

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F17%3A10328304" target="_blank" >RIV/00216208:11140/17:10328304 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0093691X16305519" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0093691X16305519</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.theriogenology.2016.11.010" target="_blank" >10.1016/j.theriogenology.2016.11.010</a>

Result language

angličtina

Original language name

Effect of sex-sorting and cryopreservation on the post-thaw sperm quality of Iberian red deer spermatozoa

Original language description

This study investigated the effect of sex-sorting and cryopreservation on post-thaw characteristics and fertility of red deer (Cervus elaphus) sperm for the first time. Semen was collected by electroejaculation from 10 mature stags during the breeding season, and each ejaculate split into four experimental groups: Bulk sorted spermatozoa, sorted but not sexed (BSS); sorted high purity X-spermatozoa (XSS); sorted high purity Y-spermatozoa (YSS); and, control non-sorted spermatozoa (NS). Following, all samples were frozen over liquid nitrogen. Two straws per stag and sample type were analyzed immediately post-thaw and following a 2-h incubation period at 37 oC. Post-thaw total motility (TM) as assessed by CASA was not different (P &lt; 0.05) among NS, BSS and YSS sperm. For XSS, post-thaw TM was lower (39%, P &lt; 0.05) than that for NS (54%) or BSS (50%), but similar (P &gt; 0.05) to that of YSS (47%) sperm. The percentage of apoptotic spermatozoa as assessed by PI/YO-PRO-1 and flow cytometry analysis, was higher (17%, P LESS-THAN OR EQUAL TO 0.05) for XSS sperm than NS (12%), BSS (13%) and YSS (14%) sperm. Following incubation there were no differences (P &gt; 0.05) in TM or percent apoptosis among treatments. Post-thaw chromatin stability calculated as the DNA fragmentation index (%DFI) was similar among treatments; following incubation %DFI increased in all except YSS, which displayed the lowest value (P &lt; 0.05). Artificial insemination of synchronized hinds yielded 44, 52 and 62% delivery rates for YSS, NS and standard frozen-thawed sperm, respectively (P &lt; 0.05). Notably, 93 and 55% of fawns born were males for the YSS and NS spermatozoa, respectively (P &lt; 0.05). In summary, Y-sorted sperm displayed acceptable post-thaw sperm evaluation parameters and the expected offspring sex ratio. More studies are needed to understand the source of sperm damage that may compromise the fertility of Y-sorted red deer sperm.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30105 - Physiology (including cytology)

Project

<a href="/en/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Theriogenology

ISSN

0093-691X

e-ISSN

—

Volume of the periodical

89

Issue of the periodical within the volume

february

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

206-213

UT code for WoS article

000392352000027

EID of the result in the Scopus database

2-s2.0-84998678102