Distinct clinicopathological features in metanephric adenoma harboring BRAF mutation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F17%3A10365237" target="_blank" >RIV/00216208:11140/17:10365237 - isvavai.cz</a>
Alternative codes found
RIV/00669806:_____/17:10365237
Result on the web
<a href="http://dx.doi.org/10.18632/oncotarget.11117" target="_blank" >http://dx.doi.org/10.18632/oncotarget.11117</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.18632/oncotarget.11117" target="_blank" >10.18632/oncotarget.11117</a>
Alternative languages
Result language
angličtina
Original language name
Distinct clinicopathological features in metanephric adenoma harboring BRAF mutation
Original language description
BRAF mutation recently has been reported in metanephric adenoma. We sought to determine the clinical and morphologic features of BRAF-mutated metanephric adenoma and to correlate BRAF mutation with BRAF V600E immunohistochemical staining results. A series of 48 metanephric adenomas and 15 epithelial-predominant nephroblastomas were analyzed for the occurrence of BRAF mutation (BRAF V600E/V600E complex, BRAF V600D, BRAF V600K and BRAF V600R) using the BRAF RGQ PCR kit (Qiagen). Immunohistochemistry was performed using monoclonal mouse antibodies against p16(INK4) and VE1 (Spring Bioscience), recognizing the BRAF V600E mutant protein. Forty-one of 48 cases (85%) showed BRAF V600E mutation; none of the other BRAF variants was detected. Of 41 BRAF-mutated metanephric adenomas, 33 showed positive VE1 immunostaining (sensitivity 80%, specificity 100%); in all cases we detected p16(INK4) expression regardless of BRAF mutation status. All epithelial-predominant nephroblastomas were BRAF-wild-type and none expressed VE1. The following features were associated with BRAF V600E mutation: older patients (p=0.01), female predominance (p=0.005) and the presence of a predominantly acinar architecture (p=0.003). In summary, BRAF-mutated metanephric adenomas were associated with older age, female predominance, and the presence of a predominant acinar component. A subset (20%) of BRAF-mutated metanephric adenomas was not detected by VE1 immunostaining.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30109 - Pathology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oncotarget
ISSN
1949-2553
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
33
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
54096-54105
UT code for WoS article
000407826100017
EID of the result in the Scopus database
2-s2.0-85029031689