Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F18%3A10383430" target="_blank" >RIV/00216208:11140/18:10383430 - isvavai.cz</a>

Result on the web

<a href="http://iopscience.iop.org/article/10.1088/2057-1739/aaa013/pdf" target="_blank" >http://iopscience.iop.org/article/10.1088/2057-1739/aaa013/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/2057-1739/aaa013" target="_blank" >10.1088/2057-1739/aaa013</a>

Result language

angličtina

Original language name

Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry

Original language description

Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established high definition single cell analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30204 - Oncology

Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Convergent Science Physical Oncology [online]

ISSN

2057-1739

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000446911400002

EID of the result in the Scopus database

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