Antimicrobial susceptibility and mechanisms of resistance of Greek Clostridium difficile clinical isolates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F19%3A10381019" target="_blank" >RIV/00216208:11140/19:10381019 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=IUUNW4WvWm" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=IUUNW4WvWm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jgar.2018.09.009" target="_blank" >10.1016/j.jgar.2018.09.009</a>

Result language

angličtina

Original language name

Antimicrobial susceptibility and mechanisms of resistance of Greek Clostridium difficile clinical isolates

Original language description

OBJECTIVES: In the present study, we examined the antimicrobial susceptibility and resistance mechanisms of Clostridium difficile recovered in Greek hospitals, during 2012-2015. METHODS: C. difficile isolates were collected from clinically-confirmed CDI from symptomatic patients in 10 Greek hospitals. MICs of various antimicrobial agents were determined by Etest. The isolates were typed by MLST. Toxin and resistance genes were detected by PCR. Chromosomal mutations in the gyrA, gyrB and rpoB genes were identified by PCR and sequencing. The genetic environment of resistance genes was characterized by Illumina sequencing. RESULTS: A total of 88C. difficile were studied. The C. difficile isolates comprised 26 STs, with ST37 (n=26) and ST11 (n=21) being the most prevalent. All isolates were susceptible to vancomycin and metronidazole, while the rates of resistance to other antimicrobials varied. 45.5% of the isolates were MDR, and the majority of them belonged to ST11 and ST37. The presence of chromosomal mutations in the gyrA, gyrB and rpoB genes was mainly observed in high risk clones, like ST11 and ST37. The resistance genes ermB, mefA, msrA and tetM were identified in different prevalence and combinations. Additionally, the cfrB and cfrC were identified for the first time in Greece, and were carried by the Tn6218 transposon and a novel plasmid, respectively. CONCLUSIONS: To our knowledge, this is the first study examining the resistant profiles and the respective mechanisms of C. difficile recovered in Greek hospitals. Gut commensals, like C. difficile, may serve as hubs for the further transfer of resistance genes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NV17-29239A" target="_blank" >NV17-29239A: Clinical Aspects of Multidrug-Resistant Infections Caused by Gram-negative Bacteria Using a Clinically Relevant Large Mammal Model of Sepsis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Global Antimicrobial Resistance

ISSN

2213-7165

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

March

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

53-58

UT code for WoS article

000461770500009

EID of the result in the Scopus database

2-s2.0-85058695307