Salivary Gland Mucinous Adenocarcinoma With Minor (Mammary Analogue) Secretory and Low-Grade In Situ Carcinoma Components Sharing the Same ETV6-RET Translocation and With No Other Molecular Genetic Aberrations Detected on NGS Analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F20%3A10414262" target="_blank" >RIV/00216208:11140/20:10414262 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ahNs400vqR" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ahNs400vqR</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1097/PAI.0000000000000806" target="_blank" >10.1097/PAI.0000000000000806</a>
Alternative languages
Result language
angličtina
Original language name
Salivary Gland Mucinous Adenocarcinoma With Minor (Mammary Analogue) Secretory and Low-Grade In Situ Carcinoma Components Sharing the Same ETV6-RET Translocation and With No Other Molecular Genetic Aberrations Detected on NGS Analysis
Original language description
In 2016, we published in this journal a hitherto undescribed composite salivary gland carcinoma arising in the left parotid gland in a 54-year-old woman. The tumor had a minor mammary analogue secretory carcinoma component (MASC), with a morphologically entirely different mucinous adenocarcinomatous component and a focal, morphologically nondescript, low-grade intraductal (in situ) component. On fluorescence in situ hybridization (FISH), w detected breaks in the ETV6 gene in all the 3 different components. However, RT-PCR failed to reveal an ETV6-NTRK3 fusion. On immunohistochemistry, the entire conventional MASC and patchy mucinous adenocarcinoma tumor cells expressed mammaglobin, while the in situ component was negative. S-100 protein was only expressed by the MASC component. Using the TruSight tumor 170 assay (Illumina, San Diego, CA) on the NextSEq. 500 sequencer (Illumina) following the usual manufacturer's protocols, we have subsequently further analyzed the in situ and invasive component (MASC mixed with mucinous adenocarcinoma components) separately. This molecular genetic study revealed the same ETV6-RET fusion (exon joining 6 to 12) in both samples. No other molecular genetic aberrations were identified in the applied panel, which consists of 170 genes. The complete list of genes and mutations covered by this assay is shown in Supplemental Table 1.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30109 - Pathology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Immunohistochemistry & Molecular Morphology
ISSN
1541-2016
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
"E54"-"E57"
UT code for WoS article
000561310100017
EID of the result in the Scopus database
2-s2.0-85072326403