Hyperbaric Oxygen Enhances Collagen III Formation in Wound of ZDF Rat
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F21%3A10436262" target="_blank" >RIV/00216208:11140/21:10436262 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=-FZjDXWjq3" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=-FZjDXWjq3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934684" target="_blank" >10.33549/physiolres.934684</a>
Alternative languages
Result language
angličtina
Original language name
Hyperbaric Oxygen Enhances Collagen III Formation in Wound of ZDF Rat
Original language description
Diabetic foot ulcer (DFU) is a serious complication of diabetes and hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. The evidence supporting the use of HBOT in DFU treatment is controversial. The aim of this work was to introduce a DFU model in ZDF rat by creating a wound on the back of an animal and to investigate the effect of HBOT on the defect by macroscopic evaluation, quantitative histological evaluation of collagen (types I and III), evaluation of angiogenesis and determination of interleukin 6 (IL6) levels in the plasma. The study included 10 rats in the control group (CONT) and 10 in the HBOT group, who underwent HBOT in standard clinical regimen. Histological evaluation was performed on the 18th day after induction of defect. The results show that HBOT did not affect the macroscopic size of the defect nor IL6 plasma levels. A volume fraction of type I collagen was slightly increased by HBOT without reaching statistical significance (1.35 +/- 0.49 and 1.94 +/- 0.67 %, CONT and HBOT, respectively). In contrast, the collagen type III volume fraction was similar to 120 % higher in HBOT wounds (1.41 +/- 0.81 %) than in CONT ones (0.63 +/- 0.37 %; p=0.046). In addition, the ratio of the volume fraction of both collagens in the wound ((I+III)(w)) to the volume fraction of both collagens in the adjacent healthy skin ((I+III)(h)) was similar to 65 % higher in rats subjected to HBOT (8.9 +/- 3.07 vs. 5.38 +/- 1.86 %, HBOT and CONT, respectively; p=0.028). Vessels density (number per 1 mm(2)) was found to be higher in CONT vs. HBOT (206.5 +/- 41.8 and 124 +/- 28.2, respectively, p<0.001). Our study suggests that HBOT promotes collagen III formation and decreases the number of newly formed vessels at the early phases of healing.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological Research
ISSN
0862-8408
e-ISSN
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Volume of the periodical
70
Issue of the periodical within the volume
5
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
12
Pages from-to
787-798
UT code for WoS article
000729810300012
EID of the result in the Scopus database
2-s2.0-85122305226