Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F25%3A10498444" target="_blank" >RIV/00216208:11140/25:10498444 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/25:43927093 RIV/62157124:16270/25:43882563 RIV/62157124:16810/25:43882563 RIV/65269705:_____/25:00082131
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=I8Mj1qxCq" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=I8Mj1qxCq</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ecoenv.2025.118320" target="_blank" >10.1016/j.ecoenv.2025.118320</a>
Alternative languages
Result language
angličtina
Original language name
Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic
Original language description
Globally, Escherichia coli is a leading cause of multiple drug-resistant (MDR) extraintestinal infections. ST131 is an MDR, pandemic sequence type that causes urinary tract and bloodstream infections in humans and companion animals, and systemic infections affecting multiple organs in poultry. ST131 has also been isolated from wastewater treatment plants and diverse wildlife sources. Studies investigating the phylogeny of ST131 from a One Health perspective are, however, limited. We performed whole-genome sequencing on 898 ST131 isolates from humans (n = 713), diverse environments (n = 139), wildlife (n = 32), food animals (n = 13), and companion animals (n = 1), sourced from the Czech Republic from 2005-2021, to identify evidence of clonal spread across different sources and to investigate carriage of genes encoding extended-spectrum beta-lactamases (ESBLs). ST131 belonging to clades C1 (262/898; 29 %) and C2 (528/898; 58.8 %) were dominant in our study. Irrespective of source or clade designation, most isolates (72.4 %) carried a blaCTX-M allele, with those residing in clade C1 carrying blaCTX-M-27, while those in clade C2 carried blaCTX-M-15. IncF plasmid sequence types were also segregated by clades, with F29:A-:B10 residing in clade A, F1:A2:B20 in clade C1, and F2:A1:B- and F31/F36:A4:B1 partitioning to clade C2. Clonal ST131 isolates (0-10 single-nucleotide polymorphisms; SNPs) were identified from humans, wild birds, and wastewater. In summary, our study showed that clonal ST131 isolates can be recovered across diverse sources in the Czech Republic, underscoring the importance of studying pandemic ExPEC lineages within a One Health context and across widespread geographic locations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Ecotoxicology and Environmental Safety
ISSN
0147-6513
e-ISSN
1090-2414
Volume of the periodical
299
Issue of the periodical within the volume
July
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
118320
UT code for WoS article
001504575400001
EID of the result in the Scopus database
2-s2.0-105005842691