Selenium activates p53 and p38 pathways and induces caspase-independent cell death in cervical cancer cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F08%3A00106812" target="_blank" >RIV/00216208:11150/08:00106812 - isvavai.cz</a>
Alternative codes found
RIV/00179906:_____/08:00001286
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Selenium activates p53 and p38 pathways and induces caspase-independent cell death in cervical cancer cells
Original language description
The mechanisms of sodium selenite-induced cell death in cervical carcinoma cells were studied during 24 h of exposure in the HeLa Hep-2 cell line. Selenite at the employed concentrations of 5 and 50 mikro mol/L produced time- and dose-dependent suppression of DNA synthesis and induced DNA damage which resulted in phosphorylation of histone H2A.X. These effects were influenced by pretreatment of cells with the SOD/catalase mimetic MnTMPyP or glutathione-depleting buthionine sulfoximine, suggesting the significant role of selenite-generated oxidative stress. Following the DNA damage, selenite activated p53-dependent pathway as evidenced by the appearance of phosphorylated p53 and accumulation of p21 in the treated cells. Concomitantly, selenite activatedp38 pathway but its effect on JNK was very weak. p53- and p38-dependent signaling led to the accumulation of Bax protein, which was preventable by specific inhibitors of p38 (SB 203580) and p53 (Pifithrin-alpha).
Czech name
Seleničitan aktivuje na p53 a p38 závislé dráhy a vyvolává na kaspázách nezávislou smrt u nádorových buněk karcinomu děložního čípku
Czech description
Byly studovány mechanismy seleničitanem vyvolané buněčné smrti u nádorové linie HeLa Hep-2 odvozené z karcinomu děložního čípku. Seleničitan v koncentracích 5 a 50 mol/l během 24 hodin vyvolal poškození DNA a aktivaci na p53 zavislé signální dráhy. Následně seleničitan aktivoval p38, ale jeho vliv na JNK byl minimální. Závěrem, seleničitan vyvolal na kaspázách nezávislou apoptózu, ale jiné mechanismy hrály také úlohu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cell Biology and Toxicology
ISSN
0742-2091
e-ISSN
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Volume of the periodical
24
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
19
Pages from-to
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UT code for WoS article
000253524700001
EID of the result in the Scopus database
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