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Gastric submucosal microdialysis in detection of rat stomach ischemia - a comparison of the 3H2O efflux technique with metabolic monitoring

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F10%3A10062016" target="_blank" >RIV/00216208:11150/10:10062016 - isvavai.cz</a>

  • Alternative codes found

    RIV/61989592:15110/10:10213168 RIV/00179906:_____/10:10062016

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Gastric submucosal microdialysis in detection of rat stomach ischemia - a comparison of the 3H2O efflux technique with metabolic monitoring

  • Original language description

    Comparison of microdialysis approaches to measure blood flow in the stomach is lacking. We compared 3H2O efflux technique with biochemical monitoring during temporary celiac artery occlusion in rats. Probes were implanted in the gastric submucosa and perfused with 3H2O; samples were analyzed for beta-activity, glucose, lactate, pyruvate and glycerol. Gastric mucosa and plasma were subjected to morphometry and analysis of myeloperoxidase, thiols and lactatdehydrogenase. The most dramatic responses were observed in lactate/pyruvate and lactate/glucose (%) ratios; the change in 3H2O efflux was less pronounced. 3H2O efflux correlated best with lactate/glucose ratio. A correlation was also found between plasma lactatdehydrogenase and relative glycerol release. Myeloperoxidase, lactatdehydrogenase and histology score were increased by ischemia-reperfusion, macroscopy and plasma thiols remained unchanged. Microdialysis metabolic monitoring is superior to the 3H2O efflux technique.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FP - Other medical fields

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2010

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Physiological Measurement

  • ISSN

    0967-3334

  • e-ISSN

  • Volume of the periodical

    31

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    14

  • Pages from-to

  • UT code for WoS article

    000281726900009

  • EID of the result in the Scopus database