Glutathione Reductase Is Inhibited by Acetaminophen-glutathione Conjugate In Vitro

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F10%3A10080783" target="_blank" >RIV/00216208:11150/10:10080783 - isvavai.cz</a>
Alternative codes found
RIV/00216275:25310/10:39883232
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Glutathione Reductase Is Inhibited by Acetaminophen-glutathione Conjugate In Vitro
Original language description
The aim of the present work was to investigate a new mechanism likely contributing to the toxic action of acetaminophen especially to explore the possible inhibition of glutathione reductase through an acetaminophen-glutathione conjugate (APAP-SG). APAP-SG conjugate was synthesized by organic synthesis and purified by column chromatography. The inhibitory effect of the conjugate on two types of glutathione reductase (from yeasts and rat hepatocytes) was tested spectrophotometrically. In addition, the enzyme acitivty (from hepatocytes lysate) was decreased to 79?7%, 67?2% and 39?7%, in 0.37, 1.48 and 3.7 mM concentration of the conjugate, respecitvely. We ound that glutathione reductase, the essential enzyme of the antioxidant system, was dosedependently inhibited by the product of acetaminophen metabolism - the conjugate of acetaminophen and glutathione.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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