Determination of pravastatin and pravastatin lactone in rat plasma and urine using UHPLC-MS/MS and microextraction by packed sorbent

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10124845" target="_blank" >RIV/00216208:11150/12:10124845 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/12:10124845

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0039914011011118" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0039914011011118</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2011.12.043" target="_blank" >10.1016/j.talanta.2011.12.043</a>

Result language

angličtina

Original language name

Determination of pravastatin and pravastatin lactone in rat plasma and urine using UHPLC-MS/MS and microextraction by packed sorbent

Original language description

A simple and reproducible method for the determination of pravastatin and pravastatin lactone in rat plasma and urine by means of ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) using deuterium labeled intemal standardsfor quantification is reported. Separation of analytes was performed on BEH C-18 analytical column (50 mm x 2.1 mm, 1.7 mu m), using gradient elution by mobile phase consisting of acetonitrile and 1 mM ammonium acetate at pH 4.0. Run time was 2 min. Quantification of analytes was performed using the SRM (selected reaction monitoring) experiment in ESI negative ion mode for pravastatin and in ESI positive ion mode for pravastatin lactone. Sample treatment consisted of a protein precipitation by ACN andmicroextraction by packed sorbent (MEPS) for rat plasma. Simple MEPS procedure was sufficient for rat urine. MEPS was implemented using the C8 sorbent inserted into a microvolume syringe, eVol hand-held automated analytical syringe and a

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

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Volume of the periodical

90

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

22-29

UT code for WoS article

000301627300003

EID of the result in the Scopus database

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