Activation of p38 MAPK and expression of TGF-beta 1 in rat colon enterocytes after whole body gamma-irradiation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10125351" target="_blank" >RIV/00216208:11150/12:10125351 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/12:43874677
Result on the web
<a href="http://informahealthcare.com/doi/pdf/10.3109/09553002.2012.654044" target="_blank" >http://informahealthcare.com/doi/pdf/10.3109/09553002.2012.654044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3109/09553002.2012.654044" target="_blank" >10.3109/09553002.2012.654044</a>

Result language
angličtina
Original language name
Activation of p38 MAPK and expression of TGF-beta 1 in rat colon enterocytes after whole body gamma-irradiation
Original language description
Purpose : To examine the p38 mitogen-activated protein kinase (p38) phosphorylation and transforming growth factor beta 1 (TGF-beta 1) expression in rat colon enterocytes after irradiation and their contribution to pathology of intestinal radiation disease. Materials and methods : Male Wistar rats were irradiated with whole body gamma-radiation of 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 Gy (Co-60, 1.44 Gy.min(-1)). Samples were taken 4 and 24 h after irradiation, immunohistochemically stained, then p38 phosphorylation and TGF-beta 1 expression were measured in apical and cryptal enterocytes using computer image analysis. In selected groups, morphometric parameters, mitosis and apoptosis were evaluated. Results : P38 phosphorylation integrated optical density (IOD)-based levels increased 2.4-fold (p {= 0.01) and 3.6 to 22.8-fold (p {= 0.001) in apical enterocytes 4 h after 0.5 Gy and 24 h after 3-10 Gy, respectively. TGF-beta 1 IOD-based expression increased 3.3- to 6.9-fold (p {= 0.001)
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/OVUOFVZ200812" target="_blank" >OVUOFVZ200812: Short-term and long-term nonspecific changes in organisms exposed to high and low doses of nerve agents on cellular and molecular level</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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