Phosphorylation of histone H2AX as an indicator of received dose of gamma radiation after whole-body irradiation of rats

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002530" target="_blank" >RIV/60162694:G44__/11:00002530 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/11:10106809
Result on the web
<a href="http://dx.doi.org/10.2754/avb201180010113" target="_blank" >http://dx.doi.org/10.2754/avb201180010113</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Phosphorylation of histone H2AX as an indicator of received dose of gamma radiation after whole-body irradiation of rats
Original language description
Rats were irradiated by 1-10 Gy of gamma radiation. Value LD50/60 was 7.37 (4.68-8.05) Gy. Histone H2AX is phosphorylated by ATM kinase on serine 139 (gamma H2AX) quickly after the irradiation. It forms microscopically visible foci in the site of doublestrand breaks of DNA. Flow-cytometric method was used for quantitative detection. This study evaluated dose-dependency of H2AX phosphorylation in peripheral lymphocytes of rats irradiated by whole-body dose 1-10 Gy. Our data show a dose-dependent increase in gamma H2AX in rat peripheral blood lymphocytes 1 h after whole-body irradiation by the dose of 1-10 Gy. We proved that phosphorylation of histone H2AX is a prompt and reliable indicator of the received radiation dose suitable for rapid measurement before the number of lymphocytes in blood starts to decrease. It can be used already 1 h after the irradiation for an estimation of the received dose of radiation. Blood can be stored in 4 degrees C for 23 h without significantly affecting
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/OVUOFVZ200806" target="_blank" >OVUOFVZ200806: Noninvasive measurement of proinflamatory markers of oxidative stress in irradiated as an indicator of received dose of radiation. Protective role of acetyl-L-carnitine</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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