Phosphorylation of histone H2AX in peripheral blood mononuclear cells after thoracic irradiation of rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002534" target="_blank" >RIV/60162694:G44__/11:00002534 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/11:10105466

Result on the web

<a href="http://dx.doi.org/10.2478/v10136-011-0007-2" target="_blank" >http://dx.doi.org/10.2478/v10136-011-0007-2</a>

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Phosphorylation of histone H2AX in peripheral blood mononuclear cells after thoracic irradiation of rats

Original language description

Lymphocytes are the most radiosensitive cells. After exposure IR die by apoptosis at a rate proportional to the dose received. We demonstrated that one hour after whole-body irradiation of rats, histone H2AX in the lymphocyte nuclei was quickly phosphorylated on serine 139. In the work presented here, we studied the kinetics of lymphocyte depletion in the blood and phosphorylation of histone H2AX in the peripheral blood lymphocytes after local (thoracic) irradiation of rats. Twenty-four hours after whole-body irradiation of the rats at a dose of 5 Gy, the lymphocyte count declined to almost zero values, whereas after local irradiation of the thorax area, the counts of lymphocytes in the peripheral blood remained unaltered. We employed flow-cytometric and microscopic gammaH2AX determination 1 h after thoracic irradiation of rats. We revealed a dose dependence on the increase in gammaH2AX in a dose range of 10-30 Gy. The microscopic method was more sensitive in the case of lower radiatio

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/OVUOFVZ200806" target="_blank" >OVUOFVZ200806: Noninvasive measurement of proinflamatory markers of oxidative stress in irradiated as an indicator of received dose of radiation. Protective role of acetyl-L-carnitine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Biomedicine - print

ISSN

1214-021X

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

209-218

UT code for WoS article

000295970900004

EID of the result in the Scopus database

—