Comparison of Resins for Metal Oxide Affinity Chromatography with Mass Spectrometry Detection for the Determination of Phosphopeptides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F13%3A10139092" target="_blank" >RIV/00216208:11150/13:10139092 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/13:43875028
Result on the web
<a href="http://www.tandfonline.com/doi/abs/10.1080/00032719.2013.773437" target="_blank" >http://www.tandfonline.com/doi/abs/10.1080/00032719.2013.773437</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparison of Resins for Metal Oxide Affinity Chromatography with Mass Spectrometry Detection for the Determination of Phosphopeptides
Original language description
Protein phosphorylation is a crucial regulatory mechanism in majority of biological processes. During MS, there is a general need to diminish suppression effect of non-phosphorylated peptides and counterbalance low abundance and insufficient ionization of phosphopeptides. Therefore, selective enrichment of their content in complex mixture has become an indispensable part of any phosphoproteomic study. In this work we employed metal oxide affinity chromatography (MOAC) approach. We have compared classicapproach of mixing TiO2 and peptides in a microtube with microcolumns - commercial tips NuTips (TiO2/ZrO2 1:1) and TopTips (R) (TiO2, TiO2/ZrO2 1:1, and ZrO2). Selectivity of the given media towards phosphopeptides was tested on a tryptic digest of mixture of bovine proteins: /-casein and fetuin (phosphoproteins) with myoglobin and bovine serum albumin (non-phosphorylated proteins) in ratio 1:1:5:5 and 1:1:50:50, respectively. After enrichment, the obtained eluates were analyzed by tande
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GPP206%2F12%2FP338" target="_blank" >GPP206/12/P338: Phosphoproteomic analysis of leukaemic cells after irradiation.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Letters
ISSN
0003-2719
e-ISSN
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Volume of the periodical
46
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
1505-1524
UT code for WoS article
000320084800004
EID of the result in the Scopus database
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