In-situ enrichment of phosphopeptides on MALDI plates modified by ambient ion landing

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10127044" target="_blank" >RIV/00216208:11310/12:10127044 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/12:00382652 RIV/61388971:_____/12:00382652 RIV/60461373:22330/12:43894151
Result on the web
<a href="http://dx.doi.org/10.1002/jms.3081" target="_blank" >http://dx.doi.org/10.1002/jms.3081</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jms.3081" target="_blank" >10.1002/jms.3081</a>

Result language
angličtina
Original language name
In-situ enrichment of phosphopeptides on MALDI plates modified by ambient ion landing
Original language description
We report substantial in-situ enrichment of phosphopeptides in peptide mixtures using titanium and zirconium dioxide-coated matrix assisted laser desorption-ionization (MALDI) plates prepared by recently reported ambient ion landing deposition technique.The technique was able to modify four common materials currently used for MALDI targets (stainless steel, aluminum, indium-tin oxide glass and polymeric anchor chip). The structure of the deposited dioxide was investigated by electron microscopy, and different surfaces were compared and discussed in this study. Two standard proteins were used to test the enrichment capabilities of modified MALDI plates: casein and in-vitro phosphorylated trehalase. The enrichment of casein tryptic digest resulted in identification of 20 phosphopeptides (including miscleavages). Trehalase was used as a suitable model of larger protein that provided more complex peptide mixture after the trypsin digestion. All four possible phosphorylation sites in treha
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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