In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F15%3A10294645" target="_blank" >RIV/00216208:11150/15:10294645 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1155/2015/476180" target="_blank" >http://dx.doi.org/10.1155/2015/476180</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2015/476180" target="_blank" >10.1155/2015/476180</a>
Alternative languages
Result language
angličtina
Original language name
In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes
Original language description
Epigallocatechin-3-gallate (EGCG) is the main compound of green tea with well-described antioxidant, anti-inflammatory, and tumor-suppressing properties. However, EGCG at high doses was reported to cause liver injury. In this study, we evaluated the effect of EGCG on primary culture of rat hepatocytes and on rat liver mitochondria in permeabilized hepatocytes. The 24-hour incubation with EGCG in concentrations of 10 ?mol/L and higher led to signs of cellular injury and to a decrease in hepatocyte functions. The effect of EGCG on the formation of reactive oxygen species (ROS) was biphasic. While low doses of EGCG decreased ROS production, the highest tested dose induced a significant increase in ROS formation. Furthermore, we observed a decline in mitochondrial membrane potential in cells exposed to EGCG when compared to control cells. In permeabilized hepatocytes, EGCG caused damage of the outer mitochondrial membrane and an uncoupling of oxidative phosphorylation. EGCG in concentratio
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oxidative Medicine and Cellular Longevity
ISSN
1942-0900
e-ISSN
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Volume of the periodical
neuveden
Issue of the periodical within the volume
March 2015
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
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UT code for WoS article
000354335800001
EID of the result in the Scopus database
2-s2.0-84928485185