A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F15%3A10320056" target="_blank" >RIV/00216208:11150/15:10320056 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038" target="_blank" >http://dx.doi.org/10.1038</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/sdata.2015.46" target="_blank" >10.1038/sdata.2015.46</a>

Result language
angličtina
Original language name
A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development
Original language description
Resurgent interest in synaptic circuitry and plasticity has emphasized the importance of 3D reconstruction from serial section electron microscopy (3DEM). Three volumes of hippocampal CA1 neuropil from adult rat were imaged at X-Y resolution of ~2 nm onserial sections of ~50-60 nm thickness. These are the first densely reconstructed hippocampal volumes. All axons, dendrites, glia, and synapses were reconstructed in a cube (~10 ?m(3)) surrounding a large dendritic spine, a cylinder (~43 ?m(3)) surrounding an oblique dendritic segment (3.4 ?m long), and a parallelepiped (~178 ?m(3)) surrounding an apical dendritic segment (4.9 ?m long). The data provide standards for identifying ultrastructural objects in 3DEM, realistic reconstructions for modeling biophysical properties of synaptic transmission, and a test bed for enhancing reconstruction tools. Representative synapses are quantified from varying section planes, and microtubules, polyribosomes, smooth endoplasmic reticulum, and endoso
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FP - Other medical fields
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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