Redefining 3Dimensional placental membrane microarchitecture using multiphoton microscopy and optical clearing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F17%3A10366361" target="_blank" >RIV/00216208:11150/17:10366361 - isvavai.cz</a>
Alternative codes found
RIV/00179906:_____/17:10366361
Result on the web
<a href="http://dx.doi.org/10.1016/j.placenta.2017.03.017" target="_blank" >http://dx.doi.org/10.1016/j.placenta.2017.03.017</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.placenta.2017.03.017" target="_blank" >10.1016/j.placenta.2017.03.017</a>
Alternative languages
Result language
angličtina
Original language name
Redefining 3Dimensional placental membrane microarchitecture using multiphoton microscopy and optical clearing
Original language description
Introduction: Remodeling of human placental membranes (amniochorionic or fetalmembrane) throughout gestation, a necessity to accommodate increasing uterine volume, involves continuous alterations (replacement of cells and remodeling of extracellular matrix). Methodologic limitations have obscured microscopic determination of cellular and layer-level alterations. This study used a combination of advanced imaging by multiphoton autofluorescence microscopy (MPAM) and second harmonic generation (SHG) microscopy along with tissue optical clearing to characterize the 3Dimensional multilayer organization of placental membranes. Methods: Placental membranes biopsies (6 mm) collected from term, not-in-labor cesarean deliveries (n = 7) were fixed in 10% formalin (native) or treated with 2,2'-thiodiethanol to render them transparent for deeper imaging. Native and cleared tissues were imaged using MPAM (cellular autofluorescence) and SHG (fibrillar collagen). Depth z-stacks captured the amnion epithelium, underlying matrix layers, and in the cleared biopsies, the decidua layer. Results: MPAM and SHG revealed fetal membrane epithelial topography and collagen organization in multiple matrix layers. Term amnion layers showed epithelial shedding and gaps. Optical clearing provided full-depth imaging with improved visualization of collagen structure, mesenchymal cells in extracellular matrix layers, and decidua morphology. Layer thicknesses measured by imaging corroborated with histology. Mosaic tiling of MPAM/SHG image stacks allowed large area visualization of entire biopsies. Conclusion: MPAM-SHG microscopy allowed for study of this multi-layered tissue and revealed shedding, gap formation, and other structural changes. This approach could be used to study structural changes associated with membranes as well as other uterine tissues to better understand events in normal and abnormal parturition.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30214 - Obstetrics and gynaecology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Placenta
ISSN
0143-4004
e-ISSN
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Volume of the periodical
53
Issue of the periodical within the volume
May
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
66-75
UT code for WoS article
000402072400011
EID of the result in the Scopus database
2-s2.0-85017021166