3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F11%3A00373200" target="_blank" >RIV/67985823:_____/11:00373200 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/11:00373200 RIV/68407700:21460/11:00185389
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging
Original language description
Nonlinear microscopy can employ two different types of signals, fluorescence and second-harmonic generation (SHG), to image biological structures with subcellular resolution. SHG imaging was used for the identification of type II collagen in an extracellular matrix. Chondrocyte proliferation was linked to production of the extracellular matrix proteins. Collagen as a typical extracellular cartilage protein was visualized, first, by CLSM using fluorescently labeled monoclonal antibodies against type II collagen. Conventional confocal microscopy images were compared with the SHG imaging in both forward and backward nondescanned modes. Well detectable signals were observed in PCL and gelatine scaffolds. Detection of collagen by SHG imaging corresponded well with collagen detection by immunohistochemical staining
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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