Changes in the Neuronal Control of the Urinary Bladder in a Model of Radiation Cystitis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F18%3A10382679" target="_blank" >RIV/00216208:11150/18:10382679 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1124/jpet.117.246371" target="_blank" >https://doi.org/10.1124/jpet.117.246371</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1124/jpet.117.246371" target="_blank" >10.1124/jpet.117.246371</a>

Result language

angličtina

Original language name

Changes in the Neuronal Control of the Urinary Bladder in a Model of Radiation Cystitis

Original language description

Currently, we have assessed the neuronal control of the urinary bladder in radiation cystitis and whether interstitial cells contribute to the condition. Fourteen days after bladder irradiation (20 Gy), rats were sedated and the urinary bladder was cut into two sagittal halves where electrical field stimulation (EFS; 5-20 Hz) was applied on the pelvic nerve afferents or stretch (80 mN) on one-half of the bladder, while contractions were registered on the contralateral half of the bladder in the absence and presence of increasing doses of imatinib (1-10 mg/kg; inhibitor of c-kit-positive interstitial cells), atropine (1 mg/kg; to block muscarinic M-3 receptors), or pyridox-alphosphate-6-azophenyl-2&apos;,4&apos;-disulfonic acid (2 mg/kg; P2(X1) purinoceptor antagonist). Urinary bladders were also excised for organ bath experiments, Western blot, quantitative polymerase chain reaction, and immunohistochemistry. In vivo, EFS contractions were enhanced after irradiation, and imatinib (1-10 mg/mg) inhibited contractions by EFS and stretched dose-dependently in controls but not in irradiated bladders. In the irradiated bladder in vitro, atropine resistance was increased and imatinib (100 mu M) inhibited contractions by EFS and agonists (ATP, methacholine) in irradiated bladders and controls. The urinary bladder expressions of P2(X1) purinoceptors, muscarinic M-3 receptor, choline acetyltransferase, c-kit, and the agonist of c-kit, stem cell factor, were not changed by irradiation. In conclusion, bladder irradiation affects several levels of neuronal control of the urinary bladder. Interstitial cells may contribute to some of the symptoms associated with radiation cystitis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Pharmacology and Experimental Therapeutics

ISSN

0022-3565

e-ISSN

—

Volume of the periodical

365

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

327-335

UT code for WoS article

000429552700013

EID of the result in the Scopus database

2-s2.0-85045023747