Effects of branched-chain amino acids on muscles under hyperammonemic conditions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F18%3A10382748" target="_blank" >RIV/00216208:11150/18:10382748 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s13105-018-0646-9" target="_blank" >https://doi.org/10.1007/s13105-018-0646-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13105-018-0646-9" target="_blank" >10.1007/s13105-018-0646-9</a>

Result language

angličtina

Original language name

Effects of branched-chain amino acids on muscles under hyperammonemic conditions

Original language description

Recent studies have indicated that ammonia plays an important role in the pathogenesis of muscle wasting in patients with liver cirrhosis and that branched-chain amino acids (BCAA; leucine, isoleucine, and valine) may act as a substrate for ammonia detoxification to glutamine (GLN). We determined the effects of enhanced availability the BCAA on ammonia detoxification to GLN and protein metabolism in two types of skeletal muscle under hyperammonemic conditions. Isolated soleus (SOL, slow-twitch) and extensor digitorum longus (EDL, fast-twitch) muscles from the left leg of white rats were incubated in medium with 1 mM ammonia (NH3 group), BCAAs at four times the concentration of the controls (BCAA group) or high levels of both ammonia and BCAA (NH3+BCAA group). The muscles from the right leg were incubated in basal medium and served as paired controls. L-[1-14C]leucine was used to estimate protein synthesis, and leucine oxidation and 3-methylhistidine release was used to evaluate myofibrillar protein breakdown. We observed decreased protein synthesis and glutamate and α-ketoglutarate (α-KG) levels and increased leucine oxidation, GLN levels, and GLN release into medium in muscles in NH3 group. Increased leucine oxidation, release of branched-chain keto acids and GLN into incubation medium, and protein synthesis in EDL was observed in muscles in the BCAA group. The addition of BCAAs to medium eliminated the adverse effects of ammonia on protein synthesis and adjusted the decrease in α-KG found in the NH3 group. We conclude that (i) high levels of ammonia impair protein synthesis, activate BCAA oxidation, enhance GLN synthesis, and decrease glutamate and α-KG levels and (ii) that increased BCAA availability enhances GLN release from muscles and attenuates the adverse effects of ammonia on protein synthesis and decrease in α-KG.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30105 - Physiology (including cytology)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Physiology and Biochemistry

ISSN

1138-7548

e-ISSN

—

Volume of the periodical

74

Issue of the periodical within the volume

4

Country of publishing house

ES - SPAIN

Number of pages

8

Pages from-to

523-530

UT code for WoS article

000452046200003

EID of the result in the Scopus database

2-s2.0-85050955506