The Effect of Flubendazole on Adhesion and Migration in SW480 and SW620 Colon Cancer Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F18%3A10383125" target="_blank" >RIV/00216208:11150/18:10383125 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/18:10383125
Result on the web
<a href="http://www.eurekaselect.com/158266/article" target="_blank" >http://www.eurekaselect.com/158266/article</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2174/1871520618666171213141911" target="_blank" >10.2174/1871520618666171213141911</a>
Alternative languages
Result language
angličtina
Original language name
The Effect of Flubendazole on Adhesion and Migration in SW480 and SW620 Colon Cancer Cells
Original language description
Background: Colon cancer is the most common type of gastrointestinal cancer. Despite advances during the last two decades, the efficacy of colorectal cancer treatment is still insufficient and new anticancer agents are necessary. Methods: In our study, colon cancer cells derived from a primary tumor (SW480) and lymph node metastasis (SW620) from the same patient were used and compared. The effect of flubendazole (FLU) on cell adhesion and migration was monitored using the x-CELLigence Real-Time Cell Analysis system. Expressions of molecules involved in adhesion and migration were analyzed using RT-PCR and western blot. Furthermore, RNA silencing of nuclear factor-kappa B in SW620 cells was used to determine the involvement of the NF-kappa B p65 regulation pathway in FLU action. Results: FLU significantly suppressed the adhesion of SW480 cells and reduced the expression of adhesion markers (ICAM-1, alpha E-catenin; beta-catenin; integrin alpha 5 and beta 1). Moreover, a significant anti-migratory potential of FLU was manifested in the SW620 cells. In addition, FLU suppressed the phosphorylation of NF-kappa B p65 and potentiated the suppression of several metastatic markers (ICAM-1, EpCAM, integrin alpha 5, beta 1, alpha-tubulin) caused by NF-kappa B p65 silencing. Conclusion: FLU has a significant anti-migratory effect in intestinal cancer cell SW480 and its lymph node metastatic cells SW620. FLU decreases the expression of some proteins involved in metastatic processes and inhibits activation of NF-kappa B p65.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Anti-Cancer Agents in Medicinal Chemistry
ISSN
1871-5206
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
837-846
UT code for WoS article
000450010700009
EID of the result in the Scopus database
2-s2.0-85057112723