Chromatographic determination of active compounds in topical formulations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124373" target="_blank" >RIV/00216208:11160/12:10124373 - isvavai.cz</a>

Result on the web

<a href="http://pubs.rsc.org/en/content/articlepdf/2011/ay/c1ay05336a" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2011/ay/c1ay05336a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c1ay05336a" target="_blank" >10.1039/c1ay05336a</a>

Result language

angličtina

Original language name

Chromatographic determination of active compounds in topical formulations

Original language description

The aim of this study was to develop and validate new methods for the determination of active compounds in topical gels. The method for the determination of nonoxinol-9 and trimecaine hydrochloride in lubricant gel by HPLC with UV detection was described. Separation was carried out using a Discovery ZR-PBD analytical column (150 x 4.6 mm, 5 mu m), and a mobile phase composed of a mixture of triethylammonium acetate buffer (pH 9; 50 mM) and methanol (22 : 78, v/v). Determination of terpinen-4-ol in lubricant gel was achieved by GC with a flame ionization detector (FID). An Alltech-AT-624 (0.32 mm ID x 30 m, 1.8 mu m film thickness) fused-silica capillary column and helium as the carrier gas, at a flow rate of 28 cm s(-1), were employed. The analysis time was less than 7.5 min for HPLC and 5 min for GC analyses. Both methods were successfully applied to the analysis of pharmaceuticals.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Methods

ISSN

1759-9660

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

1525-1529

UT code for WoS article

000304768600010

EID of the result in the Scopus database

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