On-line coupling of molecularly imprinted solid phase extraction with liquid chromatography for the fast determination of coumarins from complex samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10365191" target="_blank" >RIV/00216208:11160/17:10365191 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S073170851730941X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S073170851730941X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2017.06.033" target="_blank" >10.1016/j.jpba.2017.06.033</a>

Result language
angličtina
Original language name
On-line coupling of molecularly imprinted solid phase extraction with liquid chromatography for the fast determination of coumarins from complex samples
Original language description
In this work, an on-line SPE-HPLC method with spectrophotometric detection was developed for the determination of coumarins in complex samples. For the on-line cleanup of samples, a molecularly imprinted polymer was packed into the column cartridge and coupled directly with HPLC (MISPE-HPLC) using a column switching system. The separation of coumarins was performed on a C18 core-shell column (100 x 4.6 mm, 5 mu m) with a mobile phase consisting of 0.3% acetic acid/acetonitrile with gradient elution at a flow-rate of 1 mLmin(-1). The total time of the whole analytical run, including the extraction step, was 13.25 min. The on-line MISPE-HPLC method was optimized and validated. The results showed good linearity (0.10-100 mu g mL(-1)) with correlation coefficients higher than 0.99. The LOD values were from 0.03 to 0.15 mu gmL(-1). The proposed method was successfully applied for analysis of real samples (Cassia cinnamon, chamomile tea, and Tokaj specialty wines) and obtained recoveries varied from 78.7% to 112.2% with an RED less than 9%.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy

Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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