A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F16%3A10326196" target="_blank" >RIV/00216208:11160/16:10326196 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007/s00216-016-9402-6" target="_blank" >http://link.springer.com/article/10.1007/s00216-016-9402-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-016-9402-6" target="_blank" >10.1007/s00216-016-9402-6</a>
Alternative languages
Result language
angličtina
Original language name
A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers
Original language description
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of 100 mu L filtered beer samples into an on-line SPE-HPLC system enabled fast and effective sample extraction including separation in less than 6 min. Preconcentration of OTA and CIT from beer samples was performed on an Ascentis Express RP C18 guard column (5 x 4.6 mm), particle size 2.7 mu m, with a mobile phase of methanol/0.5 % aqueous acetic acid pH 2.8 (30:70, v/v) at a flow rate of 2.0 mL min(-1). The flow switch from extraction column to analytical column in back-flush mode was set at 2.0 min and the separation was performed on the fused-core column Ascentis Express Phenyl-Hexyl (100 x 4.6 mm), particle size 2.7 mu m, with a mobile phase acetonitrile/0.5 % aqueous acetic acid pH 2.8 in a gradient elution at a flow rate of 1.0 mL min(-1) and temperature of 50 A degrees C. Fluorescence excitation/emission detection wavelengths were set at 335/497 nm. The accuracy of the method, defined as the mean recoveries of OTA and CIT from light and dark beer samples, was in the range 98.3-102.1 %. The method showed high sensitivity owing to on-line preconcentration; LOQ values were found to be 10 and 20 ng L-1 for OTA and CIT, respectively. The found values of OTA and CIT in all tested light, dark and wheat beer samples were significantly below the maximum tolerable limits (3.0 mu g kg(-1) for OTA and 2000 mu g kg(-1) for CIT) set by the European Union.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA15-10781S" target="_blank" >GA15-10781S: On-line hyphenation of automated extraction processes with liquid chromatography for complete sample analysis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
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Volume of the periodical
408
Issue of the periodical within the volume
12
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
3319-3329
UT code for WoS article
000374302900027
EID of the result in the Scopus database
2-s2.0-84961219508