A high-performance liquid chromatography method with pre-column derivatization for the simultaneous determination of reduced glutathione, carnosine and taurine

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F14%3A10282243" target="_blank" >RIV/00216208:11160/14:10282243 - isvavai.cz</a>
Result on the web
<a href="http://pubs.rsc.org/en/content/articlehtml/2014/ay/c3ay42200c" target="_blank" >http://pubs.rsc.org/en/content/articlehtml/2014/ay/c3ay42200c</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c3ay42200c" target="_blank" >10.1039/c3ay42200c</a>

Result language
angličtina
Original language name
A high-performance liquid chromatography method with pre-column derivatization for the simultaneous determination of reduced glutathione, carnosine and taurine
Original language description
A new gradient reversed-phase HPLC method with pre-column derivatization was developed and validated for simultaneous determination of reduced glutathione, carnosine and taurine in new pharmaceutical eye drops. Chromatographic separation was achieved with a fused core analytical column Supelco Ascentis Express C18 (100 mm x 4.6 mm, 2.7 mu m). A 30 mM triethylamine in acetonitrile with 30 mM triethylamine aqueous solution (pH 2.5, adjusted with glacial acetic acid) was used in a gradient elution mode ata flow rate of 0.8 mL min(-1). The column temperature was maintained at 30 degrees C, the injection volume of the derivatized sample was 5 mu L and the detection wavelength was 375 nm. The tested validation parameters included system suitability, accuracy, precision, linearity, LOD, LOQ and sample solution stability. The standard calibration curves showed good linearity with correlation coefficients r > 0.997 for all compounds. The mean percentage recoveries obtained for reduced glutathi
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/EE2.3.20.0235" target="_blank" >EE2.3.20.0235: Establishment of Research Team Focused on Experimental and Applied Biopharmacy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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