A UHPLC method for the rapid separation and quantification of phytosterols using tandem UV/Charged aerosol detection - A comparison of both detection techniques
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10362174" target="_blank" >RIV/00216208:11160/17:10362174 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0731708517305216" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0731708517305216</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2017.03.057" target="_blank" >10.1016/j.jpba.2017.03.057</a>
Alternative languages
Result language
angličtina
Original language name
A UHPLC method for the rapid separation and quantification of phytosterols using tandem UV/Charged aerosol detection - A comparison of both detection techniques
Original language description
The presented work describes the development and validation of a rapid UHPLC-UV/CAD method using a core-shell particle column for the separation and quantitative analysis of seven plant sterols and stanols. The phytosterols (ergosterol, brassicasterol, campesterol, fucosterol, stigmasterol, and beta-sitosterol) and the phytostanol stigmastanol were separated and analyzed in 8.5 min. The sample pre-treatment procedure was optimized to be less time-consuming than any other published method, especially due to no need of derivatization, evaporation and even reconstitution step. The chromatographic separation was performed on the Kinetex 1.7 mu Phenyl-hexyl column (100 x 2.1 mm) with a mobile phase acetoni-trile/water according to the gradient program at a flow rate of 0.9 mL min(-1) and a temperature of 60 degrees C. A tandem connection of PDA and CAD (Corona Charged Aerosol Detector) was used and both detection techniques were compared. The method was validated using saponification as a first step in sample pretreatment and an universal CAD as the detector. Recoveries for all analyzed compounds were between 95.4% and 103.4% and relative standard deviation ranged from 1.0% to 5.8% for within-day and from 1.4% to 6.7% for between-day repeatability. The limits of detection were in the range of 0.4-0.6 mu g mL(-1) for standard solutions and 0.3-1.2 mu g mL(-1) for phytosterols in real samples. Although several gradient programs and different stationary phases were tested, two compounds, campesterol and campestanol, were not separated. Their peak was quantified as a sum of both analytes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085
e-ISSN
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Volume of the periodical
140
Issue of the periodical within the volume
June
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
274-280
UT code for WoS article
000402850500033
EID of the result in the Scopus database
2-s2.0-85016828304