Optimisation of an HPLC method for the simultaneous determination of pyrantel pamoate, praziquantel, fenbendazole, oxfendazole and butylhydroxyanisole using a phenyl stationary phase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124375" target="_blank" >RIV/00216208:11160/12:10124375 - isvavai.cz</a>

Result on the web

<a href="http://pubs.rsc.org/en/content/articlepdf/2012/ay/c2ay05847b" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2012/ay/c2ay05847b</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c2ay05847b" target="_blank" >10.1039/c2ay05847b</a>

Result language

angličtina

Original language name

Optimisation of an HPLC method for the simultaneous determination of pyrantel pamoate, praziquantel, fenbendazole, oxfendazole and butylhydroxyanisole using a phenyl stationary phase

Original language description

A novel and simple isocratic reversed phase HPLC method with UV and fluorimetric detection for the simultaneous determination of the active anthelmintic components pyrantel pamoate, praziquantel, fenbendazole, its degradation product oxfendazole, and theantioxidant butylhydroxyanisole (BHA) has been developed and validated. The chromatography was performed using a Phenomenex Luna 3 mm phenyl-hexyl column (150 x 3.0 mm), and the mobile phase was composed of 0.5% triethylamine at pH 9.0 and acetonitrile55 : 45 (v/v) at a flow rate of 1.0 ml min(-1). The UV detection was performed at 290 nm for pyrantel, oxfendazole and fenbendazole and at 220 nm for praziquantel. For BHA, fluorescence detection was used with excitation and emission wavelengths of 287 nm and 322 nm, respectively. The method was validated and was applied for the determination of active compounds in various dosage forms of veterinary formulations.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Methods

ISSN

1759-9660

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

1592-1597

UT code for WoS article

000304768600020

EID of the result in the Scopus database

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