Development and validation of a rapid HPLC method for the determination of ascorbic acid, phenylephrine, paracetamol and caffeine using a monolithic column

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124662" target="_blank" >RIV/00216208:11160/12:10124662 - isvavai.cz</a>

Result on the web

<a href="http://pubs.rsc.org/en/content/articlepdf/2012/ay/c2ay05784k" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2012/ay/c2ay05784k</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c2ay05784k" target="_blank" >10.1039/c2ay05784k</a>

Result language

angličtina

Original language name

Development and validation of a rapid HPLC method for the determination of ascorbic acid, phenylephrine, paracetamol and caffeine using a monolithic column

Original language description

This article reports a fast and simple liquid chromatographic method for determination of ascorbic acid, phenylephrine, paracetamol and caffeine. Salicylic acid was used as internal standard. The analytes were successfully separated in less than 5 min byisocratic elution using monolithic column, Onyx Monolithic C18 (100 x 4.6 mm), with mobile phase composed of acetonitrile and phosphate buffer (pH 6.50) (10 : 90, v/v) at a flow rate of 1.0 mL min(-1) and 25 degrees C, sample volume was 10 mu L. Detection was observed at two wavelengths 210 nm (phenylephrine, paracetamol and salicylic acid) and 235 nm (ascorbic acid and caffeine). The optimized method was applied for the determination of the analytes in pharmaceutical formulation Coldrex tablets (Smithkline Beecham Consumer Healthcare, United Kingdom) commonly used in virosis treatment.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Methods

ISSN

1759-9660

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

1588-1591

UT code for WoS article

000304768600019

EID of the result in the Scopus database

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