High-resolution monolithic columns-a new tool for effective and quick separation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10145741" target="_blank" >RIV/00216208:11160/13:10145741 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007/s00216-012-6561-y/fulltext.html" target="_blank" >http://link.springer.com/article/10.1007/s00216-012-6561-y/fulltext.html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-012-6561-y" target="_blank" >10.1007/s00216-012-6561-y</a>
Alternative languages
Result language
angličtina
Original language name
High-resolution monolithic columns-a new tool for effective and quick separation
Original language description
This work describes a comparison of three types of commercial high-performance liquid chromatography silica monolithic columns with different inner diameters and generations of monolithic sorbent: a "classic" monolithic column, the first generation (Onyx(TM) monolithic C-18, 100 mm x 4.6 mm, Phenomenex); a "narrow" monolithic column for fast separation at lower flow rates (Chromolith (R) Performance RP-18e, 100 mm x 3 mm, Merck); and a recently introduced "high-resolution" monolithic column, the next generation (Chromolith (R) HighResolution RP-18e, 100 mm x 4.6 mm, Merck). Separation efficiency (number of theoretical plates, height equivalent to a theoretical plate and van Deemter curves), working pressure, the symmetry factor and resolution were critical aspects of the comparison in the case of the separation of ascorbic acid, paracetamol and caffeine. The separations were performed under isocratic conditions with a mobile phase consisting of 10:90 (v/v) acetonitrile-phosphoric acid
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0235" target="_blank" >EE2.3.20.0235: Establishment of Research Team Focused on Experimental and Applied Biopharmacy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
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Volume of the periodical
405
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
2255-2263
UT code for WoS article
000315043800015
EID of the result in the Scopus database
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