Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00048590" target="_blank" >RIV/00216224:14110/10:00048590 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/10:00173121

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Original language description

A simple and rapid method for determination of six lignans found in plant cell cultures of Schisandra chinensis was developed and validated. The lignans were extracted from plant samples with methanol and the extracts were effectively cleaned by solid-phase extraction using Strata C18-E (Phenomenex) cartridges. Chromatographic separation was carried out on a Chromolith Performance RP-18e monolithic column (100 x 4.6 mm, Merck) using an isocratic mobile phase of acetonitrile and water in a 50:50 (v/v) ratio. The eluent was monitored at 220 nm. The base line separation of schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N and wuweizisu C was achieved in a relatively short time period (20 minutes), which was made possible by the relatively high flow rate of the mobile phase (2 ml/min). The lower limit of quantitation was 0.1 mg/l for schizandrin and gomisin A, 0.3 mg/l for deoxyschizandrin, gamma-schizandrin, and gomisin N and 1 mg/l for wuweizisu C.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA522%2F07%2F0995" target="_blank" >GA522/07/0995: Regulation of secondary metabolites biosynthesis in cell culture of Schisandra chinensis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOMEDICAL CHROMATOGRAPHY

ISSN

0269-3879

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

9

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

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UT code for WoS article

000281424600007

EID of the result in the Scopus database

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