UHPLC in Modern Bioanalysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124923" target="_blank" >RIV/00216208:11160/12:10124923 - isvavai.cz</a>
Result on the web
<a href="http://pubs.rsc.org/en/Content/Chapter/9781849735490-00237/978-1-84973-549-0" target="_blank" >http://pubs.rsc.org/en/Content/Chapter/9781849735490-00237/978-1-84973-549-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/9781849735490-00237" target="_blank" >10.1039/9781849735490-00237</a>

Result language
angličtina
Original language name
UHPLC in Modern Bioanalysis
Original language description
UHPLC (ultra-high performance liquid chromatography) has become an indispensable tool in the field of bioanalysis over the past years due to high separation efficiency, selectivity and sensitivity, which are very important features for analysis of targetanalyte(s) in complex matrices. In spite of several detection approaches available, UHPLC-MS/MS using triple quadrupole analyzer remains the most important technique in quantitative bioanalysis. Recently, also UHPLC-MS with high-resolution mass spectrometry (HRMS) has been applied in both quantitative and qualitative bioanalytical approaches. Basic features, applications, benefits and drawbacks of both approaches are discussed besides less commonly used detection approaches such as UV or FD (fluorescence detection). Sample preparation step is the most important part of bioanalytical method as it impacts all the later steps of the assay. An appropriate technique should be chosen according to extraction time, selectivity, number of steps
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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